TABLE 1.

Type and sampling location of marine sediment samples investigated and MPN counts for selected samples

Sample no.	Sample type (length, cm)	Location	Coordinates	Depth (m)	MPN (107 cells g−1) in:		Ratio of MPN at 2°C to MPN at 25°C	Viable-cell recovery (%)b
					SWN	M2216a
10GC01c	Core (21)	George V Basin	66°32′S, 143°38′E	761
10GB01	Grab	George V Basin	66°32′S, 143°38′E	750	2.50	0.59	2.8	2.2
13GB02C	Core (14)	Mertz Drift	66°33′S, 143°5′E	864
13GB02	Grab	Mertz Drift	66°33′S, 143°4′E	864	2.96	0.48	4.1	2.6
25GB13B	Core (13)	Mertz Drift	66°34′S, 143°0′E	843
25GB13	Grab	Mertz Drift	66°34′S, 143°0′E	843	0.92	0.08	1.6	1.7
27GB15C	Core (14)	Mertz Drift	66°31′S, 143°23′E	793
27GB15	Grab	Mertz Drift	66°31′S, 143°23′E	793	4.75	0.32	2.0	3.8
28GB16C	Core (14)	North Mertz Drift	66°23′S, 143°19′E	739
28GB16	Grab	North Mertz Drift	66°23′S, 143°19′E	739	1.75	0.35	2.1	2.1
28GB17	Grab	North Mertz Drift	66°24′S, 143°19′E	739	2.45	0.27	2.5	2.9
29GB18B	Core (15)	North Mertz Drift	66°21′S, 143°18′E	709
29GB18	Grab	North Mertz Drift	66°21′S, 143°18′E	709	1.65	0.27	1.7	2.6
Casey1	Grab	O'Briens Bay	66°16′S, 110°33′E	25
Casey2	Grab	O'Briens Bay	66°16′S, 110°32′E	30
Burton	Grab	Burton Lake, Vestfold Hills	66°38′S, 78°8′E	16

^aM2216, M2216 marine liquid medium (diluted in an equal volume of ASW).

^bThe total direct count used for calculating the recovery percentage is the averaged value from the top 4-cm layer of the corresponding sediment core sample.

^cThis core was subjected to extensive clone library analysis (8) with GenBank entries AF424054 to AF424538 and AF425747 to AF425762.