TABLE 1.

Parameters of linear regressions of manual versus automated counting at different thresholds of the FISH image

FISH threshold (% avg gray value)	Slope	Regression parameter y intercept	r2
110	0.825	22.61	0.939
120	1.020	2.91	0.987
130	1.025	−0.39	0.989
140	1.012	−1.44	0.988
150	0.994	−1.78	0.987
160	0.978	−1.87	0.986
170	0.963	−1.88	0.984
180	0.947	−1.80	0.983
190	0.934	−1.73	0.982
200	0.929	−2.28	0.984