Table 1.
The secondary structure of lysozyme dissolved in water and in glycerol as determined from the far-UV CD spectra
| Component | Secondary structure content, %
|
|
|---|---|---|
| Water | Glycerol | |
| α-Helix | 30 | 30 |
| β-Sheet | 13 | 21 |
| β-Turn | 27 | 25 |
| Unordered | 30 | 24 |
CD spectra were measured at 25°C. Lyophilized lysozyme was dissolved in water (pH 3.8) at 15 mg/ml, and then diluted 1:100 either by the same aqueous solution or by glycerol. The secondary structure contents were determined as outlined in Materials and Methods.