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. 2003 May;69(5):2857–2863. doi: 10.1128/AEM.69.5.2857-2863.2003

TABLE 2.

Flow-cytometric analyses of cultures subjected to different storage conditionsa

Bacterium % of cells correctly interpretedb after incubation for:
14 days at 5°C 24 h at −18°C
Gram-positive strains
    B. cereus 80 (15) 83 (6)
    L. lactis 89 (2) 100 (2)
    M. luteus 100 (1) 99 (2)
    S. aureus 85 (6) 100 (0)
    S. agalactiae 100 (0) 100 (1)
    S. dysgalactiae 100 (1) 100 (1)
    S. uberis 100 (1) 100 (1)
Gram-negative strains
    E. cloacae 76 (7) 98 (1)
    E. coli 97 (3) 100 (1)
    K. oxyloca 77 (8) 100 (1)
    P. fluorescens 58 (4) 82 (6)
    P. putida 68 (8) 98 (0)
Avg 86 96
a

The results were obtained by using the procedure described in “Gram-staining technique for bacterial cultures.”

b

Average of two determinations. Standard deviations are given in parentheses.