Figure 6.

Interaction between glibenclamide and MgADP or MgATP on the photoaffinity labeling of SUR1. Membrane proteins from COS-7 cells expressing SUR1 were preincubated with 10 μM 8-azido-[α-³²P]ATP. After free 8-azido-[α-³²P]ATP was removed, proteins were incubated with 3 mM MgSO₄ (□), with MgSO₄ and 0.5 mM ADP (●), or with MgSO₄ and 0.5 mM ATP (○) in the presence of various concentrations of glibenclamide for 15 min at 37°C. Proteins were then photoaffinity-labeled. Photoaffinity labeling is expressed as percentage of control (photoaffinity-labeled immediately).