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Location of pghP on an EcoRV fragment and the amino-terminal region of PghP. Deduced and determined amino-terminal sequences match perfectly (underline). Possible ribosome-binding site (GGAGG) precedes initiation ATG codon at 9 bp upstream. Nucleotides 5′-TA(C/T)CCGAA(C/T)ATTGA(A/G)GC-3′ used in Southern blotting and in cloning pghP correspond to region between residues 7 and 12 (boxed) of deduced amino-terminal sequence.
