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Hemolytic activity of four S. pyogenes strains on Ba plus cholesterol medium after 18 h of incubation at 37°C in 5% CO2 in air. Strains C203U (SLS− SLO+), Blackmore (SLS+ SLO−), M25 (streptin producer), and Tn216 (Tn916 inhibitor negative derivative of strain M25) are indicated.