TABLE 1.
Primers used in this study
| Primer | Sequence | Accession no. and locationa | Restriction siteb |
|---|---|---|---|
| P1 | 5′GCAAGCTTCGTAAGAGAAAAGCGAAACGGATGT3′ | AF467001, 2823-2850 | HindIII |
| P2 | 5′TGCGCTAGCTGTGAAGCGAACTTTCTTCTTGT3′ | AF467001, 3516-3493 | NheI |
| P3 | 5′CGGGTACCAATCAAAGAGGCTGGGCTTC3′ | AF467001, 5040-5059 | KpnI |
| P4 | 5′AAGAATTCGCAAAATGTGGTCGCAGTGCCA3′ | AF467001, 5565-5544 | EcoRI |
| ermA | 5′GTCGGATCCATTAAGAAGGAGGGATTCGTCA3′ | M11180, 489-510 | BamHI |
| ermB | 5′GTTGGTACCGAATTATTTCCTCCCGTTAAAT3′ | M11180, 1453-1432 | KpnI |
a
Location coordinates correspond to the primer sequence portion shown in italics.
b
Restriction site corresponds to the underlined portion of the primer sequence.