TABLE 1.
Primer | DNA sequence (5′ to 3′) |
---|---|
pILL550- T3-F4-PstI | TAA CTG CAG AGA ACT AGG ACA CGA AAG AGC |
pILL550-T7- F2-EcoRI | TTT GAA TTC GTC TTA GCA TTA TCG TTG G |
tetO-F-PstI | TAA CTG CAG AGA TTC AGT ATT ATA ACA AGG |
tetO-R-PstI | TTA CTG CAG CAT CAT AAT TAT CTC TAA TCC |
ompE-prom- F-XhoI | TTT CTC GAG CTT TAG ATG TTT TTA TCT TCG |
ompE-prom- R-HinDIII | CGA AAG CTT TCT CCT TGT CAA AAA TTA ATA AAA C |
gfp-F-HinDIII | TTT AAG CTT AGT AAA GGA GAA GAA CTT TTC ACT GG |
gfp-R-BgIII | TTT AGA TCT TTT TGA CAC CAG ACA AGT TG |