Table 1.
ALLN inhibits cAMP-induced adipocyte differentiation
| Treatment | Relative extent of
differentiation
|
|
|---|---|---|
| −ALLN | +ALLN | |
| None | − | − |
| MDI | ++++ | − |
| MIX | +++ | − |
| DEX | ++ | ++ |
| INS | + | + |
| dibutyryl-cAMP | +++ | − |
| Forskolin | +++ | − |
Two-day postconfluent 3T3-LI preadipocytes were treated or not (None) for 2 days with MDI, MIX, DEX, INS, 100 μM dibutyryl-cAMP (cAMP) or 100 μM forskolin in the absence or presence of 26 μM ALLN. The extent of differentiation, estimated by Oil Red O staining of cytoplasmic triglyceride (+ symbols indicate the relative extent of cytoplasmic triglyceride accumulation) was assessed on day 7, (−) symbol indicates <5% differentiation by day 7. Results are representative of three individual experiments.