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Southern hybridization analysis of P. flocculosa (WT) PF-1 chromosomes and deficient mutants with 32P-labeled pSceI-Hyg, separated by CHEF gel electrophoresis. Lanes 1 to 6 correspond to mutants PF78, PF79, PF84, PF88, PF96-1, and PF100-1, respectively.
