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Bioassay of 80% MeOH:20% H2O fraction prepared from P. flocculosa (WT) PF-1 and transformants with Phomopsis sp. A 200-μl volume of each of the following fractions were bioassayed: control (Czapek Dox medium with hygromycin B) (A), WT (B), transformant PF6 from group I (C), and transformant PF96-1 from group IV (D). Agar plugs inoculated with Phomopsis sp. were placed 2 cm from the center of each well, and results were recorded following incubation for 3 days at 22°C.
