FIG. 4.

Alignment of pITS-2 band sequences representing C. parvum type 1 over 182 positions (a) and of pITS-2 sequences representing C. parvum type 2 over 175 positions (b), determined following PCR-based enrichment from SSCP bands. The sequences of the bands are presented in the same order (top to bottom) as in Fig. 3. A dot indicates a base identical to that in the top sequence, a dash indicates an insertion-deletion event, and an asterisk indicates sequence identity among all sequences of a particular genotype. Sequences representing C. parvum type 1 (codes H1 and P18 [25]) and C. parvum type 2 (codes type A and type B [22]) have been included for comparative purposes.