TABLE 1.
Results from SSCP analyses of amplicons produced from 102 C. parvum DNA samples purified from oocyst isolates from humans returning to the United Kingdom following recent travel to different countriesa
Profile | Travel destination (no. of samples) | No. of samples | |
---|---|---|---|
pSSU | pITS-2 | ||
1 | 1A | Spain (18), Greece (2), Cyprus (3), Egypt (1), Pakistan (4), United States (1), Jamaica (1), Cuba (1), Peru (1), Mexico (1) | 33 |
1B | Denmark (1), France (2), Spain (18), Cyprus (2), Greece (2), Turkey (3), Pakistan (3), India (1), Mexico (2), Carribean Islands (1) | 35 | |
1C | Kenya (1), Gambia (1) | 2 | |
1D | Kenya (1) | 1 | |
1E | Pakistan (1) | 1 | |
1F | Pakistan (2), United States (1) | 3 | |
1G | Nigeria (1), Pakistan (2), India (1), Caicos Islands (1), Equador (1) | 6 | |
1H | Spain (2) | 2 | |
1I | Jamaica (1) | 1 | |
1J | Spain (2), Greece (1) | 3 | |
1K | Menorca (1) | 1 | |
2 | 2A | Spain (3) | 3 |
2B | Spain (1) | 1 | |
2C | Portugal (2) | 2 | |
2D | Portugal (1), Uganda (1), Saudi Arabia (1) | 3 | |
2E | Africa (1) | 1 | |
2F | Cyprus (1)b | 1 | |
2G | Turkey (1) | 1 | |
2H | Pakistan (1) | 1 | |
2I | Mexico (1) | 1 |
Samples were categorized to the genotypic level (types 1 and 2) based on pSSU profiles and to the subgenotypic level (1A to 1K and 2A to 2I) based on pITS-2 profiles.
This sample (c35) from Cyprus differed slightly in profile from all other type 2 samples examined; this difference related to one A-to-T transversion in the pSSU sequence.