Nucleotide sequence of the PLA depolymerase gene. The deduced amino acid sequence is shown below the nucleotide sequence. A putative promoter region (−10 and −35), a Shine-Dalgarno (S.D.) sequence, and a ρ-independent terminator (horizontal arrows) are indicated. A sequence that exhibits the characteristics of a signal peptide is underlined, and the putative active site is doubly underlined. SphI and SmaI restriction sites at the beginning and end of the sequence, respectively, are underlined.