TABLE 1.
Conserved sequences in family I-4 and I-5 lipases
| Enzyme | Amino acid sequencea | Identity (%) | Accession no. |
|---|---|---|---|
| PlaA | HIVAHSMGGANS | AB093482 | |
| Family I-4 lipases | |||
| B. pumilus | DIVAHSMGGANT | 50.3 | A34992 |
| B. licheniformis | DIVAHSMGGANT | 51.4 | CAB95850 |
| B. subtilis LipA | DIVAHSMGGANT | 49.0 | M74010 |
| B. subtilis LipB | DIVAHSMGGANT | 44.9 | C6952 |
| Geotrichum candidum | DIVAHSMGGANT | 46.2 | A02813 |
| Consensus sequenceb | dIVAHSMGGANt | ||
| Family I-5 lipases | |||
| B. thermoleovorans | HIIAHSQGGQTA | 20.0 | AF134840 |
| B. thermocatenulatus | HIIAHSQGGQTA | 21.6 | X95309 |
| B. stearothermophilus | HIIAHSQGGQTA | 20.5 | U78785 |
| Consensus sequencec | -I-AHSQGG--- | ||
| P. lemoignei PhaZ7 | DIVAHSMGVSMS | AY026355 |
a
The consensus pentapeptide is shown in bold type.
b
Consensus sequence for PlaA and family I-4 lipases. Residues conserved only in family I-4 lipases are indicated by lowercase letters.
c
Consensus sequence for PlaA, family I-4 lipases, and I-5 lipases.