TABLE 2.
Substrate specificity of purified PLA depolymerase
| Substrate | Relative activity |
|---|---|
| Polyestersa | |
| PLA05 | ++ |
| PLA10 | ++ |
| PLA15 | + |
| PLA20 | + |
| PBSA3001 | ++ |
| PBSA3020 | ++ |
| PBS1001 | + |
| PBS1020 | + |
| PCL | ++ |
| PES | ++ |
| P(HB-co-HV) | − |
| Triglyceridesb | |
| Triolein | + |
| Tributyrin | + |
| p-Nitrophenyl estersc | |
| C2 | 0.60 |
| C4 | 1 |
| C8 | 0.79 |
| C10 | 0.56 |
| C16 | 0.50 |
| C18 | 0.27 |
a
Decrease in the turbidity of the emulsion of >60% (++) or <60% (+) or no decrease (−) after incubation at 37°C for 1 h.
b
+, NaOH consumption on titration of between 0.1 and 0.2 ml.
c
Relative specific activies, with the value of the C4 ester set at 1.