FIG. 3.

(A to C) T-RFLP analysis of clone PeH59 (affiliated with the CFB phylum) amplicons after restriction digestion with different enzymes, resulting in the expected T-RFs only (MspI [A]) or in the formation of pseudo-T-RFs (AluI [B] and HhaI [C]). (D) 16S rRNA gene secondary structure of clone PeH59 as predicted by the mfold software including the sequence stretches around detected pseudo-T-RFs. RS, restriction sites. Bold numbers indicate restriction sites with corresponding T-RFs in the electropherogram. RFU, relative fluorescence units.