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Schematic model of pseudo-T-RF formation. (A) PCR-related parameters influencing the formation of partly single-stranded amplicons. (B) Involvement of the secondary structure of partly single-stranded amplicons in the formation of pseudo-T-RFs. dsDNA, double-stranded DNA; ssDNA, single-stranded DNA; solid triangles, restriction site cut (MspI); open triangle, restriction site not cut.
