Table 2.
E7-dependent alterations of intracellular concentrations of glycolytic metabolites in 14/2 cells
| Metabolites |
± SEM
|
||
|---|---|---|---|
| E7 off | E7 on | Significance | |
| FBP, nmole/mg protein | 13.4 ± 0.6 | 28.3 ± 5.9 | P < 0.5 |
| PEP, nmole/mg protein | 0.9 ± 0.1 | 1.7 ± 0.2 | P < 0.05 |
| IMP, nmole/mg protein | 0.2 ± 0.4 | 2.0 ± 1.7 | P < 0.05 |
| [ATP] : [ADP] | 5.2 ± 1.4 | 3.5 ± 0.6 | P < 0.05 |
 |
4.1 ± 0.9 | 1.9 ± 0.2 | P < 0.001 |
 |
19.3 | 6.8 | |
The concentration of various metabolites is given for mock-treated and dexamethasone-treated 14/2 cells. In each case, the significance of dependence on E7 expression was calculated as described (8, 12). n = 5. The term (pyruvate⋅ATP):(PEP⋅ADP) was calculated from the concentrations of the individual metabolites.