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ATP dependence of the interaction between N272 and insulin. The effects of N272 (10 μM) on the aggregation of insulin B-chain (50 μM) (●) were monitored in the absence (○) and presence of 10 mM ATP and 20 mM MgCl2 (■) or 150 μM GA (▵). Addition of MgATP/GA to a preformed N272⋅insulin complex showed no detectable effect in this assay (▴).
