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Dissociation constants of Hsp90 fragments for ADP and ATP
| Fragment |
Kd, μM
|
|
|---|---|---|
| ADP | ATP | |
| In the absence of peptide GR1 | ||
| N210 | 40 ± 2 | 98 ± 4 |
| N272 | 43 ± 3 | 94 ± 1 |
| In the presence of peptide GR1 | ||
| N210 | ND | 101 ± 6 |
| N272 | ND | 287 ± 27 |
The dissociation constants of ADP/ATP and N210/N272 were determined as described in the text and the legend of Fig. 2 in the absence or presence of GR1 (500 μM). The numbers represent the obtained dissociation constants with their calculated errors. ND, not determined.
