FIG. 2.

Comparison between the reference curves of the HHV-8 and calibrator real-time PCR assays. The number of the pVU56 (HHV-8) and pVU46 (calibrator) molecules is indicated on the x axis, and the cycle of threshold (Ct) is indicated on the y axis. Open triangle, TaqMan assay for HHV-8; closed circles, TaqMan assay for calibrator. The equations for the HHV-8 and the calibrator constructs were y = 39.052 − 3.333 log (x) and y = 39.084 − 3.340 log (x), respectively. The solid lines were obtained by linear regression analysis of the data (r² > 0.99; P < 0.001), and dotted lines indicate the 95% confidence intervals for the regression.