TABLE 4.
Results of various analyses of EV typing
| Accession no. | No. of samples | Resulta according to: |
Highest identity score (%) |
Geographical location of prototype (yr) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| LBM pool method | IFA | PCR with Coxprim primer set | RFLP analysis | PCR VP1 (187-222) | Nucleo- tide | Amino acid | Prototype | |||
| AJ309241 | 1 | Echovirus 15 | Echovirus 4 | − | − | + | 100 | 100 | Coxsackievirus A16 (Af177911) | Taiwan (1998) |
| AJ309245 | 1 | Echovirus 25 | + | + | NS | + | 96 | 97 | Echovirus 25 (Af107282) | Germany (1998) |
| AJ309248 | 1 | Echovirus 30 | Echovirus 30 | + | NS | + | 99 | 99 | Echovirus 30 (Af067071) | Germany (1997) |
| AJ309249 | 1 | Echovirus 30 | Echovirus 30 | − | − | + | 92 | 98 | Echovirus 30 (Af128027) | United States (1983) |
| AJ295171 | 5 | Echovirus 30 | Echovirus 30 | + | NS | + | 99 | 100 | Echovirus 30 (e30241456) | France (1998) |
| AJ309247 | 2 | Echovirus 30 | Echovirus 30 | + | NS | + | 99 | 100 | Echovirus 30 (e30241456) | France (1998) |
| AJ309273 | 1 | Echovirus 30 | + | + | NS | + | 94 | 100 | Echovirus 30 (e30241456) | France (1998) |
| AJ309246 | 1 | Echovirus 30 | Echovirus 30 | + | NS | + | 97 | 100 | Echovirus 30 (e30241456) | France (1998) |
| AJ309257b | 1 | − | Coxsackievirus B3 | + | Coxsackie- virus B4 | + | 100 | 100 | Coxsackievirus B3 (CB3VC02) | United States |
| AJ309256 | 1 | Coxsackie- virus A9 | Coxsackievirus A9 | + | NS | + | 99 | 98 | Echovirus 13 (AY007223) | Germany (2000) |
| AJ309254b | 1 | − | Echovirus | − | − | + | 83 | 83 | Echovirus 8 (Af081325) | United States |
| AJ309251 | 1 | Echovirus 9 | + | + | NS | + | 82 | 84 | Echovirus 8 (Af081325) | United States |
| AJ309255 | 1 | Echovirus 3 | + | + | NS | + | 83 | 95 | Echovirus 8 (Af081325) | United States |
| AJ309253 | 2 | Coxsackie- virus B4 | Echovirus | + | NS | + | 82 | 97 | Echovirus 8 (Af081325) | United States |
| AJ309242b | 1 | − | + | + | NS | + | 94 | 99 | Echovirus 3 (Af081316) | United States |
| AJ309243 | 2 | Echovirus 9 | Echovirus 9 | + | Echovirus 9 | + | 94 | 100 | Echovirus 3 (Af081316) | United States |
| AJ309263 | 1 | Echovirus 13 | + | + | NS | + | 95 | 98 | Echovirus 9 (X92886) | Germany |
| AJ309259b | 1 | − | Coxsackievirus B2 | − | − | + | 86 | 97 | Coxsackievirus B2 (Af295491) | Sweden (1984) |
| AJ309260b | 1 | − | + | − | − | + | 86 | 99 | Coxsackievirus B2 (Af295491) | Sweden (1984) |
| AJ309262 | 1 | Coxsackie- virus B4 | Coxsackievirus B4 | + | NS | + | 96 | 99 | Coxsackievirus B4 (Af160005) | France (1996) |
| AJ309261 | 1 | − | Coxsackievirus B4 | − | − | + | 99 | 98 | Coxsackievirus B4 (Af160005) | France (1996) |
| AJ309258 | 1 | Echovirus 30 | Coxsackievirus B1 | + | − | + | 84 | 91 | Coxsackievirus B1 (M16560) | United States (1949) |
a
+, positive result; −, negative result; NS, not significant.
b
This strain was also tested by primer set 011-012 of the VP1 region (see Table 5).