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Relative PDEδ binding of GST-RPGR392 mutants. PDEδ (1 μM) and 3 μM of WT or mutant GST-RPGR392 were incubated with GSH-Sepharose at 20°C and 30°C for 30 min. The GSH-Sepharose bound protein complexes were applied onto a SDS/PAGE after washing with PBS. Band intensities of the PDEδ were determined by densitometric software. The intensity of the PDEδ bound to WT-RPGR392 was set to 100%.
