TABLE 1.

Chronological dissemination of T. gondii in experimental mouse infection demonstrated by PCR and histology^a

Technique	No. positive/no. analyzed at h postinfection:
	6	12	18	24	36	48	72
PCR	Negative	Negative	Ascites 2/5	Ascites 4/5, blood 4/5	Liver 2/5, ascites 4/5, heart 3/5, blood 4/5, lung 3/5	Liver 2/5, ascites 4/5, heart 4/5, blood 5/5, lung 4/5	Liver 2/4, ascites 3/3, heart 3/4, blood 3/3, lung 3/4, brain 3/4
Histology	Negative	Negative	Negative	Negative	Negative	Heart 2/5	Liver 1/4, heart 2/4, lung 2/4

^aNegative, PCR or histology gave negative results for the detection of T. gondii parasites. Liver, brain, heart, and lung were analyzed by both PCR and histology at each time, and ascites and blood samples were analyzed only by PCR. Tissues not listed in the table were negative. Although five mice were sacrificed at each time point, one mouse died before 72 h, and one sample each of blood and ascites were destroyed.