TABLE 1.
Results of real-time PCR versus those of culture
| Sample no. | Cta | PCR resultb | Culture resultc | % Cultivatable |
|---|---|---|---|---|
| 1 | 25.1 | 5.5 × 107 | 4.5 × 106 | 8.2 |
| 2 | 31.7 | 6.5 × 105 | 1.9 × 104 | 2.9 |
| 3 | 32.2 | 4.8 × 105 | 1.0 × 103 | 0.2 |
| 4 | 34.4 | 1.1 × 105 | 2.0 × 103 | 1.8 |
| 5 | 23.2 | 1.9 × 108 | 5.4 × 105 | 0.3 |
| 6 | 30.5 | 1.5 × 106 | 9.0 × 103 | 0.6 |
| 7 | 28.6 | 5.3 × 106 | 2.8 × 104 | 0.5 |
| 8 | 35.5 | 5.3 × 104 | 0 | 0 |
| 9 | 31.4 | 7.8 × 105 | 3.1 × 105 | 40 |
| 10 | 35.0 | 7.5 × 104 | 0 | 0 |
| 11 | 26.1 | 2.8 × 107 | 3.6 × 105 | 1.3 |
| 12 | 22.7 | 2.8 × 108 | 6.8 × 106 | 2.4 |
Ct values were used for calculation of CFU equivalent.
CFU equivalent based on real-time PCR.
CFU of E. coli.