Figure 3.

Functional complementation of the Moco-deficient E. coli mogA mutant by heterologous gephyrin expression. The columns show nitrate reductase activity of the crude protein extract derived from E. coli wild-type (wt) strain RK4353 (filled bar), untransfected E. coli mogA cells grown in the presence (+Mo) or absence (−Mo) of 1 mM sodium molybdate (stippled bars), and mogA mutant cells (RK5231, gray bars) transformed with the control plasmid pcDNA3 (−) without insert, expressing the full-length gephyrin cDNA clone P1 (+geph), the C-terminal truncated gephyrin (+gephΔC), and Cnx1 from Arabidopsis (+cnx1).