Table 1.

Cell-free translation and transfection analysis of Gtx-luciferase fusion constructs

Construct	% complementarity to 18S rRNA		Luciferase activity assayed
			By cell-free translation		By transfaction
	nt. 701–741	nt. 1104–1136	P19	C6	P19	C6
Gtx-w	78	67	100	100	100  ±  9	100  ±  2
Gtx-d	(38)*	(43)*	477  ±  46	300  ±  57	398  ±  9	252  ±  22
Gtx-m1	73	61	208  ±  36	230  ±  50	196  ±  1	370  ±  12
Gtx-m2	66	100	53  ±  7	41  ±  10	40  ±  18	55  ±  33
Gtx-m3	100	61	204  ±  27	148  ±  37	448  ±  16	354  ±  2

For the various constructs, the percentage complementarity to two sites within the 18S rRNA are indicated, including GU base-pairs, along with the activity as measured by luciferase activity. Constructs were assayed in cell-free lysates prepared from P19 or C6 cells and after transfection into P19 or C6 cells. nt., Nucleotides. Luciferase activity was measured as raw light units, and the values were normalized to the activity of construct Gtx-w. The luciferase activity for each construct was calculated from at least three experiments, performed in duplicate. Data are the mean ± SEM. 

^*Construct Gtx-d contains a 42-nucleotide deletion of the Gtx 5′UTR. The numbers in parentheses are the best complementary matches to the new junction, comparing up to 50 nucleotides to either side of the junction.