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Real-time amplification plot of 18 representative clinical samples. Positive control DNA from C. diphtheriae (NCTC 10648 toxigenic) and three clinical samples amplified early (20 to 25 cycles). The majority of samples amplified late (>34 cycles) in the 40-cycle program. +, Positive control DNA; Ct, cycle threshold (i.e., the cycle at which a statistically significant increase in fluorescence is first detected).
