TABLE 2.
Type, reference, and standard strains (n = 44) representing respiratory pathogens and flora used in the evaluation of specificity and sensitivity of the real-time PCR assays for detection of the tox gene
| Speciesa | Isolate | Real-time PCR assay result |
|---|---|---|
| C. accolens | CDC1455 | − |
| C. diphtheriae | NCTC 10648 (tox positive) | + |
| C. diphtheriae | NCTC 10356 (tox negative) | − |
| C. diphtheriae | NCTC 3984 (weakly toxigenic) | + |
| C. jeikeium | CDC1457 | − |
| C. minutissimum | CDC536 | − |
| C. mycetoides | CDC1460 | − |
| C. pseudodiphtheriticum | CDCG2486 | − |
| C. striatum | CDC530 | − |
| C. ulcerans | NCTC 12077 | − |
| C. xerosis | NCTC 12078 | − |
| H. haemolyticus | CDC3442 | − |
| H. influenzae, biogroup aegyptius | CDC5575 | − |
| H. influenzae, nontypeable | CDC4420 | − |
| H. influenzae serotype a | CDC3956 | − |
| H. influenzae serotype b | CDC3957 | − |
| H. influenzae serotype c | CDC3958 | − |
| H. influenzae serotype d | CDC3959 | − |
| H. influenzae serotype e | CDC3960 | − |
| H. influenzae serotype f | CDC3961 | − |
| H. parainfluenzae | CDC3438 | − |
| M. catarrhalis | CDC4419 | − |
| M. catarrhalis | M6452 | − |
| N. cinerea | M6451 | − |
| N. gonorrhoea | M6450 | − |
| N. lactamica | M6454 | − |
| N. meningitidis non groupable | CDC4631 | − |
| N. meningitidis serogroup A | CDC318 | − |
| N. meningitidis serogroup B | CDC321 | − |
| N. meningitidis serogroup C | CDC323 | − |
| N. meningitidis serogroup W135 | CDC327 | − |
| N. meningitidis serogroup Y | CDC326 | − |
| N. meningitidis serogroup Z | CDC329 | − |
| N. meningitidis serogroup Z′ | CDC330 | − |
| N. sicca | M6453 | − |
| N. subflava | M6449 | − |
| S. aureus | ATCC 1258 | − |
| S. aureus | ATCC 25923 | − |
| Streptococcus group A | CDC2373-96 | − |
| Streptococcus group B | CDCSS615 | − |
| Streptococcus group C | CDCSS498 | − |
| Streptococcus group D | CDCSS1344 | − |
| Streptococcus group G | CDCSS175 | − |
| Streptococcus pneumoniae | ATCC 49619 | − |
a
C., Corynebacterium; H., Haemophilus; M., Moraxella; N., Neisseria; S., Staphylococcus.