Fig. 3.

Temperature-mediated modulation of the effects of viscosity on the H⁺-ATPase. The V_max was calculated in the presence of different trehalose concentrations and temperatures as described in methods. The relative V_max (V_max0/V_max) was plotted against the relative viscosity (η/η0). η0 is the viscosity of the solution in the absence of trehalose and V_max0 is the V_max in the absence of trehalose. Temperatures (°C): ○ 20, ● 35 and □ 40. The solid lines are linear regressions of the data.