TABLE 1.

RV primer and probe sequences

Oligonucleotide	Sequenceb	Positionc	Function
Primer
Rhino-P1.1 pd 904a	5′ AATTCTAATACGACTCACTATAGGGAGAGAGCACACGGGGGTCTTCACA 3′	239-258	Downstream primer group B RV
Rhino-P1.5 EG 59	5′ AATTCTAATACGACTCACTATAGGGAGAGAGCACACGGGGCTCTTC 3′	242-258	Downstream primer group B RV
Rhino-P2.1 pd 906a	5′ AGACCTGGCAGATGAGGCT 3′	131-149	Upstream primer group B RV
Rhino-P1.2 pd 905a	5′ AATTCTAATACGACTCACTATAGGGAGAGAGCACACGGGGCTCTTCACA 3′	239-258	Downstream primer group A RV
Rhino-P1.6 pd 1275	5′ AATTCTAATACGACTCACTATAGGGAGAGAGCTCAGTGGGCTCTTC 3′	242-258	Downstream primer group A RV
Rhino-P2.2 pd 907a	5′ TAGTCTGGTCGATGAGGCT 3′	131-149	Upstream primer group A RV
Rhino-P2.3 pd 1274	5′ TAGCTTAGGCTGATGAGTC 3′	131-149	Upstream primer group A RV
OL-26d	5′ GCACTTCTGTTTCCCC 3′		RT-PCR primer
OL-27d	5′ CGGACACCCAAAGTAG 3′		RT-PCR primer
Probe
Rhino-BIO1 pd 908a	5′ CCCCACTGGCGACAGTGT 3′	157-174	Biotin capture probe group B RV
Rhino-BIO3 pd 1147	5′ CCCCACTGGYRACAGTGK 3′	157-174	Biotin capture probe group B RV
Rhino-N1 pd 910a	5′ AGCCTGCGTGGCTGCCTGC 3′	178-196	ECL probe group B RV
Rhino-BIO2 pd 909a	5′ CCCCACGGGCGACCGTGT 3′	157-174	Biotin capture probe group A RV
Rhino-BIO8 pd 1277	5′ CCCCACTGGTGACAGTGG 3′	157-174	Biotin capture probe group A RV
Rhino-N2 pd 911a	5′ AGCCTGCGTGGCGGCCAGC 3′	178-196	ECL probe group A RV
Rhino-N5 pd 1276	5′ AGGCTGCGTTGGGGCCTAC 3′	178-196	ECL probe group A RV

^aPrimers and probes used initially, based on published sequences of the rhinovirus 5′ NCRs.

^bSingle-letter code: R, A or G; Y, C or T; K, G or T.

^cPositions based on numbering of Mori et al. (27).

^dData from Gama et al. (10.).