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Method for preparation of in vitro-transcribed RNA containing PRRSV viral primer sequences. (A) Schematic of the relative oligonucleotide primer sequence positions, synthesis steps, and time required for each step. (B) Approximately 1 μg of in vitro-transcribed RNA (lane 1) following denaturing agarose gel electrophoresis with ethidium bromide staining. Lane M, RNA molecular size markers.
