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. 2003 May;41(5):2055–2061. doi: 10.1128/JCM.41.5.2055-2061.2003

FIG. 4.

FIG. 4.

Detection of endogenous RT-PCR inhibitors in samples submitted for diagnostic assays. Prior to PRRSV RT-PCR, 100 molecules of competitor RNA were added to RNA extracted from six swine serum samples. The results of PRRSV RT-PCR following agarose gel electrophoresis for samples from which viral RNA was extracted with Trizol (lanes 1 to 6) or Qiagen (lanes 7 to 12) columns are shown. The results for negative (no template) and positive (PRRSV RNA) control reactions are shown in lanes 13 and 14, respectively. Lane M, DNA molecular size markers.