TABLE 1.
Detection of RT-PCR inhibitors in diagnostic laboratory accessions
| Sample | No. (%) of samples
|
||
|---|---|---|---|
| Total | Partially inhibitedb | Completely inhibitedc | |
| Tissue poola | 91 | 13 (14.3) | 8 (8.8) |
| Brain | 28 | 8 (28.6) | 1 (3.6) |
| Serum (accession A) | 173 | 16 (9.2) | 5 (2.9) |
| Serum (accession B) | 61 | 23 (37.7) | 14 (23.0) |
| Whole blood | 117 | 6 (5.1) | 3 (2.6) |
| Total | 470 | 66 (14.0) | 31 (6.6) |
a
A combination of lung, lymph nodes, tonsil, and/or spleen tissue.
b
Partial inhibition was defined as a markedly reduced intensity of the competitor RNA RT-PCR product following agarose gel electrophoresis when 100 copies of competitor RNA were added to the reaction mixture.
c
Complete inhibition was defined as the absence of the competitor RT-PCR product following agarose gel electrophoresis when 100 copies of competitor RNA were added to the reaction mixture.