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Typical IS1311 PCR-REA patterns with undigested and HinfI-digested amplicons in adjacent lanes. Shown are bovine M. avium subsp. paratuberculosis (lanes 1 and 2), ovine M. avium subsp. paratuberculosis (lanes 3 and 4), and PCR-negative control (lanes 5 and 6) isolates and a bovine isolate with several mutations resulting in multiple restriction sites (lanes 7 and 8).
