Figure 3.

Effects of SSF1, CNS1, and HCH1 on p60^v-src activity in E381K cells. E381K cells expressing an empty vector or a vector expressing HSP82, SSF1, CNS1, or HCH1 were transformed with a galactose-inducible p60^v-src expression vector. Cells grown to mid-logarithmic phase at 25°C in raffinose-containing medium were shifted to galactose-containing medium for 6 hours to induce expression of p60^v-src. Total cellular proteins were isolated, separated on 7.5% SDS/PAGE gels and transferred to Immobilon-P membranes. (A) Equal sample loading was confirmed by Coomassie blue staining. (B) p60^v-src activity was assessed by reacting the blot with an antibody specific for phosphotyrosine. (C) p60^v-src accumulation was assessed by reacting the blot with an antibody specific for p60^v-src.