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Phosphorylation of Y845 is essential for EGFR function. K+ cells were transfected with plasmid DNA encoding Y845F (Y-F) or wt EGFR, cultured for 2 days, serum starved for 30 hr, and left untreated or treated with 40 ng/ml EGF, 10% fetal bovine serum, or 10 μM LPA for 18 hr. Results are expressed as the mean percent ± SEM of cells expressing EGFR that were positive for BrdUrd incorporation. Thirty-five to 100 cells were analyzed for each variable in three independent experiments.
