TABLE 1.
Origin, serotype, molecular type, and PLB1 RFLP pattern of C. neoformans strains used in this study
| Strain | Origin | Serotype | Molecular typea | RFLP profile
|
|
|---|---|---|---|---|---|
| AvaI | HindIII | ||||
| Cryptococcus neoformans var. grubii | |||||
| BL-1 | Lung, Australia | A | VNI | A1 | H1 |
| CN042 | CSF, New Zealand | A | VNI | A1 | H1 |
| H99 | CSF, USA | A | VNI | A1 | H1 |
| LA190 = 76(a) | Pigeon droppings, Spain | A | VNI | A1 | H1 |
| LA193 = 6(a) | Pigeon droppings, Spain | A | VNI | A1 | H1 |
| LA194 = I-01 | CSF, Colombia | A | VNI | A1 | H1 |
| LA195 = I-21 | CSF, Colombia | A | VNI | A1 | H1 |
| LA199 = I-50 | CSF, Colombia | A | VNI | A1 | H1 |
| WM 148 | CSF, Australia | A | VNI | A1 | H1 |
| CN037 | CSF, New Zealand | A | VNII | A2 | H1 |
| CN038 | CSF, New Zealand | A | VNII | A2 | H1 |
| CN039 | CSF, New Zealand | A | VNII | A2 | H1 |
| CN041 | CSF, New Zealand | A | VNII | A2 | H1 |
| LA327 = MLP-1473 | CSF, Brazil | A | VNII | A2 | H1 |
| WM 626 | CSF, Australia | A | VNII | A2 | H1 |
| AD hybrid | |||||
| WM 328 = 88-1358 | No data | AD | VNIII | A3 | H2 |
| WM 329 = 90-7031 | No data | AD | VNIII | A3 | H2 |
| LA 184 = 2(c) | CSF, Spain | AD | VNIII | A3 | H2 |
| LA 185 = 3(c) | CSF, Spain | AD | VNIII | A3 | H2 |
| LA 192 = 4(a) | Pigeon droppings, Spain | AD | VNIII | A3 | H2 |
| LA 317 = 138027 | Blood, Argentina | AD | VNIII | A3 | H2 |
| RKI-A208 | Pigeon droppings, Germany | AD | VNIII | A3 | H2 |
| RKI-M1644/90 | Clinical, Germany | AD | VNIII | A3 | H2 |
| RKI-M364/98 | Clinical, Germany | AD | VNIII | A3 | H2 |
| WM 339 = RU-27809 | No data | AD | VNIII | A3 | H2 |
| TBS 28 | Clinical, India | AD | VNIII | A3 | H2 |
| TBS 54 | Clinical, India | AD | VNIII | A3 | H2 |
| WM 628 | CSF, Australia | AD | VNIII | A3 | H2 |
| Cryptococcus neoformans var. neoformans | |||||
| B3501 = ATCC 52817 | CSF, USA | D | VNIV | A4 | H3 |
| B-87455 | Blood, Australia | D | VNIV | A4 | H3 |
| CN3501 = ATCC 52817 | CSF, USA | D | VNIV | A4 | H3 |
| RKI-318 | Clinical, Germany | D | VNIV | A4 | H3 |
| RKI-1089/89 | Clinical, Germany | D | VNIV | A4 | H3 |
| RKI-M186/99 | Clinical, Germany | D | VNIV | A4 | H3 |
| TP0416a | Eucalyptus debris, Australia | D | VNIV | A4 | H3 |
| WM 629 | Blood, Australia | D | VNIV | A4 | H3 |
| Cryptococcus neoformans var. gattii | |||||
| LA174 = 48(a) | Goat, Spain | B | VGI | A5 | H4 |
| LA175 = 50(a) | Goat, Spain | B | VGI | A5 | H4 |
| LA176 = 52(a) | Goat, Spain | B | VGI | A5 | H4 |
| LA177 = 54(a) | Goat, Spain | B | VGI | A5 | H4 |
| LA178 = 56(a) | Goat, Spain | B | VGI | A5 | H4 |
| LA179 = 58(a0 | Goat, Spain | B | VGI | A5 | H4 |
| LA220 = I-628 | CSF, Colombia | B | VGI | A5 | H4 |
| SAVIM | No data, Papua New Guena | B | VGI | A5 | H4 |
| WM 276 = TSC/SC1 | Eucalyptus debris, Australia | B | VGI | A5 | H4 |
| WM 179 | CSF, Australia | B | VGI | A5 | H4 |
| WOWOPA | No data, Papua New Guena | B | VGI | A5 | H4 |
| LA196 = I-34 | CSF, Colombia | B | VGII | A6 | H4 |
| LA221 = I-638 | CSF, Colombia | B | VGII | A6 | H4 |
| LA222 = I-642 | CSF, Colombia | B | VGII | A6 | H4 |
| LA230 = I-357 | CSF, Colombia | B | VGII | A6 | H4 |
| LA231 = I-414 | CSF, Colombia | B | VGII | A6 | H4 |
| ATCC 32608 = NIH191 | CSF, USA | C | VGII | A6 | H4 |
| ARN1 | Eucalyptus debris, Australia | B | VGII | A6 | H4 |
| WM 178 | Lung, Australia | B | VGII | A6 | H4 |
| ATCC 34880 | CSF, USA | C | VGIII | A7 | H4 |
| CN043A | CSF, New Zealand | C | VGIII | A7 | H4 |
| CN0438 | CSF, New Zealand | C | VGIII | A7 | H4 |
| LA205 = I-78 | CSF, Colombia | C | VGIII | A7 | H4 |
| LA234 = I-559 | Almond tree, Colombia | C | VGIII | A7 | H4 |
| LA235 = I-737 | Almond tree, Colombia | C | VGIII | A7 | H4 |
| LA237 = I-871 | Almond tree, Colombia | C | VGIII | A7 | H4 |
| LA382 = BV19 | CSF, Venezuela | C | VGIII | A7 | H4 |
| UCLA380 | No data, USA | C | VGIII | A7 | H4 |
| UCLA385 | No data, USA | C | VGIII | A7 | H4 |
| WM 161 | Eucalyptus debris, USA | B | VGIII | A7 | H4 |
| WM 728 | Eucalyptus depris, USA | B | VGIII | A7 | H4 |
| B5242 | Clinical, India | B | VGIV | A8 | H5 |
| V869 | Urine, South Africa | C | VGIV | A8 | H5 |
| V709 | Urine, South Africa | C | VGIV | A8 | H5 |
| M27055 | Urine, South Africa | C | VGIV | A8 | H5 |
| MCS34 | CSF, Thailand | C | VGIV | A8 | H5 |
| WM 779 | Cheata, South Africa | C | VGIV | A8 | H5 |
a
Molecular type as determined by PCR-fingerprinting with the minisatellite specific primer M13 (21).