TABLE 1.

Oligonucleotide probes and targeted microbial groups

Probe name	Full namea	Sequence (5′ to 3′)	% G+C	Major target group	Reference
UNIV907	S-*-Univ-0907-a-A-22	CCC CGT CAA TTC CTT TGA GTT T	45	All life	3
UNIV1390	S-*-Univ-1390-a-A-18	GAC GGG CGG TGT GTA CAA	61	All life	28
EUB336	S-D-Bact-0336-a-A-18	TGC CTC CCG TAG GAG TCT	61	Domain Bacteria	4
EUB338	S-D-Bact-0338-a-A-18	GCT GCC TCC CGT AGG AGT	67	Domain Bacteria	2
EUB927	S-D-Bact-0927-a-A-17	ACC GCT TGT GCG GGC CC	76	Domain Bacteria	7
PLA46	S-P-Planc-0046-a-A-18	GAC TTG CAT GCC TAA TCC	50	Planctomycetes	18
EUB338II	S-*-BactP-0338-a-A-18	GCA GCC ACC CGT AGG TGT	67	Planctomycetes	6
EUB338III	S-*-BactV-0338-a-A-18	GCT GCC ACC CGT AGG TGT	67	Verrucomicrobia	6
ALF968	S-Sc-aProt-0968-a-A-18	GGT AAG GTT CTG CGC GTT	56	α-Proteobacteria	17
BET42ab	L-Sc-bProt-1027-a-A-17	GCC TTC CCA CTT CGT TT	53	β-Proteobacteria	1515
GAM42ab	L-Sc-gProt-1027-a-A-17	GCC TTC CCA CAT CGT TT	53	γ-Proteobacteria	15
ACID20	S-Div-Acido-0020-a-A-19	CAG CGT TGA TTC TGA GCC A	53	Acidobacteria	This study
ACID294	S-*-Acido-0294-a-A-17	CCG TGC GCC CTC TCA GG	76	Groups of Acidobacteriac	This study
ACID351	S-*-Acido-0351-a-A-20	GAA CAA TTC CCC ACT GCT GC	55	Groups of Acidobacteriad	This study
ACID1399	S-Div-Acido-1399-a-A-20	TTT CGT GAT GTG ACG GGC GG	60	Acidobacteria	This study
GPOS1192	S-*-Grps-1192-a-A-22	TAA GGG GCA TGA TGA TTT GAC G	45	Gram-positive bacteria	This study
GPOS1199	S-*-Grps-1192-a-A-15	TAA GGG GCA TGA TGA	47	Gram-positive bacteria	This study
HGC69ab	L-Div-Hgc-1901-a-A-18	TAT AGT TAC CAC CGC CGT	50	Actinobacteria	19
LGC353a	S-Div-Lgc-0353-a-A-16	AAG ATT CCC TAC TGC T	44	Low G+C gram-positive bacteria	This study
LGC353b	S-Div-Lgc-0353-b-A-18	GGA AGA TTC CCT ACT GCT	50	Low G+C gram-positive bacteria	This study
BACIL1136	S-G-Bacil-1136-a-A-22	AGT CAC CTT AGA GTG CCC AAC T	50	Bacillus	This study
NEG E7		GAT GAT GAT GAT GAT GA	35	Negative control	S. Bavykin, unpublished data

^aFull names have been standardized by Alm et al. (1).

^b23S rRNA probes.

^cEnvironmental clone MC22, MC103, and Acidobacterium capsulatum groups.

^dEnvironmental clone MB1228 and RB40 groups.