TABLE 1.
C. testosteroni bacterial strains and plasmids
| Strain or plasmid | Characteristicsa | Source or reference |
|---|---|---|
| Bacterial strains | ||
| TA441 | Wild type, Tesb | 2 |
| TesB− | tesB::Kmr mutant of TA441 | This work |
| TesD− | tesD::Kmr mutant of TA441 | This work |
| TesE− | tesE::Kmr mutant of TA441 | This work |
| ORF11− | ORF11::Kmr mutant of TA441 | This work |
| ORF12− | ORF12::Kmr mutant of TA441 | This work |
| Plasmids | ||
| pUC19 | Apr; lacZ | 20 |
| pC29 | Apr; pSuperCosI with BamHI insert of TA441 | This work |
| pC26 | Apr; pSuperCosI with BamHI insert of TA441 | This work |
| pC29E3 | Apr; pUC19 with EcoRI insert from pC29 | This work |
| pC26ScH | Apr; pUC19 with SacI-HindIII insert from pC26 | This work |
| pORF11-Kmr | Apr; pC29E3 derivative with Kmr gene in NruI site of ORF11 | This work |
| pORF12-Kmr | Apr; pC29E3 derivative with Kmr gene in PstI site of ORF12 | This work |
| pTesD-Kmr | Apr; pC29E3 derivative with Kmr gene in NspI site of tesD | This work |
| pTesE-Kmr | Apr; pC29E3 derivative with Kmr gene in SmaI site of tesE | This work |
| pTesD | Apr; pC29E3 derivative coding tesD | This work |
| pTesE | Apr; pC29E3 derivative coding tesE | This work |
a
Apr, ampicillin resistance.
b
Grown on testosterone as the sole carbon source.