TABLE 2.

HPLC analysis of the culture media of the gene-disrupted mutants incubated with testosterone in C medium for 24 h^a

Compound	RT (min)	Relative peak area (%) of accumulated intermediate compounds with:
		TA441	TesB−	ORF11−	ORF12−	TesD−	TesE−	TesD−c
X5	4.1	NDb	ND	ND	ND	100	45	156
X1	6.5	ND	0.56	3.9	28	23	0.56	12.3
ADD	7.4	ND	3.3	31	13.9	23	0.56	2.8
Tsd	8.2	ND	20	2.8	3.9	3.9	2.2	0.56
4AD	9.5	ND	10.6	2.8	8.4	2.8	ND	0.56

^aData are presented with relative peak area (X5 in strain TesD⁻ as 100%) of each compound detected at 210 nm.

^bND, not detected.

^cGrown in LB+C medium with 0.1% testosterone.

^dTs, testosterone.