TABLE 1.

Identification of proteins by peptide fingerprinting

Protein designation	Localization	Level of expressiona	Mol wt (103)	Isoelectric point	Matching translated gene and corresponding proteinb	Protein accession no.	No. of matching peptides
Proteins involved in energetic metabolism or protein synthesis
NC-4	Cytoplasmic	Constant	50.1	5.4	tuf1, protein synthesis elongation factor EF-Tu	2021268A	8
NI-3	Cytoplasmic	Induced	95.5	5.3	idh, isocitrate dehydrogenase	CAB88977	12
NI-5	Cytoplasmic	Induced	58.9	5.2	atpA, ATP synthase alpha chain	CAB94542	11
NI-13	Cytoplasmic	Induced	35.0	6.1	tsf, guanine-nucleotide exchange factor EF-Ts	031213	8
NI-14	Cytoplasmic	Induced	38.0	6.1	fba, fructose biphosphate aldolase	Q9X8R6	8
NI-22	Cytoplasmic	Induced	80.0	5.7	tkt3, transketolase	T36007	6
Proteins involved in the acquisition of carbon
NI-19	Cytoplasmic	Induced	31.0	5.7	SC1G7.03, putative epoxide hydrolasec	CAC37878	3
NI-20	Cytoplasmic	Induced	28.5	4.8	fkb1, acyl-CoA dehydrogenase	AAF86388	8
NI-21	Cytoplasmic	Induced	46.0	4.9	SC4C6.23, putative transcription regulator (cellulose degradation)d	T35031	3
Proteins involved in growth under stress conditions
NI-7	Cytoplasmic	Induced	55.0	4.8	groEL2, stress-induced chaperonin	CAB93056	9
NI-31	Cytoplasmic	Induced	15.0	4.9	groES, stress-induced chaperonin	P40172	6
NR-8	Cytoplasmic	Repressed	61.7	4.1	SCC80.05c, putative cell division trigger factore	CAC09996	14
XI-26	Extracellular	Induced	30.0	5.3	sodF2, FeSOD	AAD33130	5
XI-27	Extracellular	Induced	28.0	5.2	sodF2, FeSOD	AAD33130	5
XI-28	Extracellular	Induced	28.0	4.5	SCC8A.26c, putative tellurite resistance proteinf	CAB92844	5
XR-25	Extracellular	Repressed	13.5	5.0	SC5C7.16, probable ATP-GTP binding proteing	T35223	4

^aLevel of expression in the presence of plants.

^bMost proteins were associated with S. coelicolor; the only exception was NI-20, which was associated with S. hygroscopicus.

^cThere was 29.6% identity in a 318-amino-acid overlap with Arabidopsis thaliana epoxide hydrolase.

^dThere was 50% identity in a 304-amino-acid overlap with S. reticuli CebR and 50% identity in a 301-amino-acid overlap with T. fusca CelR.

^eThere was 35.9% identity in a 412-amino-acid overlap with B. subtilis trigger factor.

^fThere was 65.8% identity in a 190-amino-acid overlap with S. marcescens tellurium resistance protein.

^gThere was 44.5% identity in a 137-amino-acid overlap with E. coli osmotically inducible protein C.