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Purification of laccase produced by S. cyaneus
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U mg of protein−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Initial supernatant, (NH4)2SO4 | 87.67 | 88.33 | 1.02 | 1 | 100 |
| Precipitation (50%), (NH4)2SO4 | 62.76 | 83.71 | 1.33 | 1.3 | 93.7 |
| Precipitation (20%) | 45.8 | 70.84 | 1.55 | 1.52 | 79.3 |
| Econo Methyl | 21.44 | 52.53 | 2.44 | 2.39 | 58.8 |
| Econo Q | 8.19 | 51.64 | 6.3 | 6.16 | 57.8 |
