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Sephadex LH-20 column chromatogram of active fractions from the eluates of preparative TLC. The Sephadex LH-20 column (26 by 950 mm) was eluted with methanol at a flow rate of 0.15 ml min−1. All fractions were bioassayed for the antifungal and antioomycete activity against C. orbiculare and P. capsici using the paper disk-agar diffusion method.
