TABLE 2.
Induction of DJ nematode release of GFP-labeled P. luminescens
| Sample | Treatment | Release activitya
|
||
|---|---|---|---|---|
| Mean % | SD (%) | n | ||
| M. sexta larva hemolymph | None | 25 | 7 | 3 |
| Incubated at 68°C for 15 min | 25 | 8 | 2 | |
| Pronase (20 μg/ml, 50°C, 2 h) | 25 | 17 | 3 | |
| EDTA (25 mM) | 32 | 2 | 2 | |
| Chelex (10 mg/ml) | 33 | 0 | 2 | |
| Centricon 10-kDa filtrate | 27 | 3 | 4 | |
| Centricon 10-kDa retentate | 10 | 1 | ||
| Melanized | 33 | 0 | 2 | |
| Dialyzed (5 kDa) | 0 | 1 | ||
| Dialyzed (10 kDa) | 0 | 1 | ||
| Diluted 1/10 | 0 | 1 | ||
| Levamisole (0.5 mM) | 12 | 6 | 4 | |
| Ivermectin (100 μg/ml, 1% DMSO) | 16 | 4 | 4 | |
| DMSO (1%, vol/vol) | 33 | 1 | ||
| Kanamycin (30 μg/ml) | 23 | 3 | 2 | |
| Other arthropod hemolymph | ||||
| G. mellonella larvae | 42 | 2 | 2 | |
| T. molitor larvae | 47 | 5 | 2 | |
| D. melanogaster larvae | 30 | 1 | ||
| Freshwater crab | 37 | 14 | 2 | |
| A. domestica (feeder cricket) | 36 | 7 | 2 | |
| Blaberus sp. (tropical cockroach) | 55 | 2 | 2 | |
| L. alienus Foerster (Ant) | 25 | 2 | 2 | |
| Insect cell culture supernatants | ||||
| A. gambiae 4a-3B | 31 | 17 | 2 | |
| T. ni TN368 | 24 | 4 | 2 | |
| T. ni TNH15 | 21 | 2 | 2 | |
| S. frugiperda SF21 | 17 | 3 | 2 | |
| D. melonogaster DL-1 | 20 | 4 | 2 | |
| Nonarthropod fluids | ||||
| Fresh human blood | 2 | 1 | 2 | |
| Culture of P. luminescens NC1/1 | 3 | 0 | 2 | |
| Peptone broth | 3 | 0 | 2 | |
| Grace's insect cell culture medium | 5 | 4 | 4 | |
| Schneider's insect cell culture medium + 10% FBS | 9 | 2 | 2 | |
| Water | 3 | 0 | 2 | |
a
Percentage of DJ nematodes containing GFP-labeled P. luminescens cells that released bacteria 60 min after incubation. The mean percentage, SD, and number of independent experiments (n) where at least 30 DJ nematodes containing GFP-labeled bacteria were observed are shown.