TABLE 1.

Steady-state kinetic constants for LinB and its mutants

Substrate	Enzyme	Sp act (μmol · s−1 · mg of protein−1)	kcat (s−1)	Km (mM)	kcat/Km (mM−1 · s−1)	Ksi (mM)
1-Chlorobutane	Wild type	0.034 ± 0.002a	1.11 ± 0.03	0.23 ± 0.02	4.83	NAb
	Q146A + D147V	0.016 ± 0.001	0.65 ± 0.02	0.06 ± 0.01	10.10	NA
	L177A	0.015 ± 0.003	0.41 ± 0.03	0.08 ± 0.02	4.99	NA
	I211L	0.008 ± 0.001	0.27 ± 0.01	0.05 ± 0.01	5.26	NA
	L248I	0.021 ± 0.002	0.76 ± 0.03	0.21 ± 0.02	3.58	NA
	F143W	0.018 ± 0.002	0.58 ± 0.03	0.14 ± 0.02	4.05	NA
1,3-Dibromopropane	Wild type	0.094 ± 0.013	40.9 ± 5.2	24.10 ± 3.23	1.70	0.49 ± 0.06
	Q146A + D147V	0.034 ± 0.001	25.8 ± 2.6	5.90 ± 1.22	4.37	0.21 ± 0.04
	L177A	0.033 ± 0.005	1.9 ± 0.2	0.06 ± 0.02	30.86	27.20 ± 11.29
	I211L	0.072 ± 0.008	3.4 ± 0.7	0.19 ± 0.11	17.68	6.82 ± 2.31
	L248I	0.095 ± 0.006	20.3 ± 4.3	5.11 ± 2.18	3.96	1.50 ± 0.52
	F143W	0.107 ± 0.003	21.5 ± 2.8	10.07 ± 2.44	2.13	1.63 ± 0.34

^aMean ± standard error.

^bNA, not applicable.