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The CRE is necessary for the activity of the cyclin D1 promoter in chondrocytes. MCT cells were transiently transfected with −66CD1LUC (wild-type promoter) or −66 mut CD1LUC (containing a mutated CRE) and pRlSV40 (for standardization). after transfection (30 h), cells were harvested, firefly luciferase activity was measured, and standardized to Renilla luciferase activity to yield the relative luciferase activity. −66 mut CD1LUC conferred 22% of the activity of −66 CD1LUC.
