Overexpression of ATF-2 increases the activity of
the cyclin D1 CRE. MCT cells were cotransfected with −66CD1LUC or −66
mut CD1LUC, either empty expression vector or an expression vector for
ATF-2, and pRlSV40. After transfection (30 h), cells were harvested,
and firefly luciferase activity was measured and standardized to
Renilla luciferase activity to yield the relative luciferase activity.
Overexpression of ATF-2 increases the activity of −66 CD1LUC 3.5-fold,
whereas it had no effect on the activity of −66 mut CD1LUC.